Gene-edited iPSC: the role of Automation in Cell Line Development

Precise gene editing is crucial for the development of cell-based therapies, particularly allogeneic approaches, where enhancing cell function, improving persistence, and reducing immunogenicity are key objectives. Developers often face the challenge of combining multiple gene knockouts with knock-ins, resulting in an average of six to eight gene edits. However, traditional manual genome editing processes are limited in their scalability, efficiency, and standardization.